Evaluation of the applicability of immunochromatography to the identification of live plague vaccines and the tularaemia allergen (Tularin)

The regulatory standards require that the identification of live plague vaccines and liquid tularaemia allergen (Tularin) should be performed by immunofluorescence. A major drawback recommended method is its labour intensive nature. However, immunochromatography represents an alternative offers a number advantages, including rapid testing easy result interpretation. aim study was to assess applicability (Tularin). Materials methods. authors tests using samples pharmacopoeia standard for vaccines, three commercial batches vaccine, two These were tested immunochromatographic assay (ICA) reagent kits detection Yersinia pestis (ICA System Y. ) Francisella tularensis F. manufactured State Scientific Center Applied Microbiology Biotechnology. Results. findings show effective, rapid, species-specific confirm presence in sample vaccine or To perform immunochromatography, recommend diluting concentration 10 9 bacterial cells/mL undiluted Conclusions. results may support inclusion ICA into as method.